Peptide Reconstitution Calculator
Enter your vial and dose information. Get precise syringe-ready instructions—no math required.
Vial Information
Desired Dose Per Injection
Mixing Method
Enter Your Values
Fill in the vial and dose info to calculate.
How to Reconstitute Peptides
Follow these steps carefully to ensure proper peptide reconstitution and maintain potency.
Clean the Vial
Before handling, use an alcohol swab to thoroughly clean the rubber stopper on both the peptide vial and the bacteriostatic water vial. Allow to air dry for a few seconds. This prevents contamination and ensures a sterile environment for reconstitution.
Equalize Pressure
Draw the same amount of air into your syringe as the volume of bacteriostatic water you plan to inject. Insert the needle into the BAC water vial and inject the air first—this equalizes pressure and makes drawing the water much easier and more accurate.
The "Slow Drip" Technique
Insert the needle into the peptide vial and aim for the glass wall—never spray directly onto the powder. Slowly depress the plunger, allowing the water to gently trickle down the side of the vial. This prevents damage to the delicate peptide structure and ensures proper dissolution.
Storage & Handling
Do not shake the vial. Gently swirl or roll it between your palms until the powder is fully dissolved and the solution is clear. Once reconstituted, store the vial in the refrigerator (36–46°F / 2–8°C). Most reconstituted peptides remain stable for 4–6 weeks when properly refrigerated.
Peptide Reconstitution Calculator & Dosage Guide
Why Use a Peptide Mixing Calculator?
Accurate dosing is critical for effective research. Most peptides (such as BPC-157, TB-500, Semaglutide, and Tirzepatide) are sold as a lyophilized (freeze-dried) powder in a vial. To use them, you must "reconstitute" the powder by mixing it with a liquid solvent, typically Bacteriostatic Water.
This calculator eliminates the guesswork. It converts the quantity of peptide (mg) and the volume of water (mL) into precise dosage units (mcg) for a standard U-100 insulin syringe.
How to Reconstitute Peptides: Step-by-Step
Follow this protocol to ensure sterility and preserve the integrity of your compounds.
Supplies Needed:
- Peptide Vial (e.g., 5mg or 10mg)
- Bacteriostatic Water (BAC Water)
- Alcohol Prep Pads
- Mixing Syringe (larger gauge)
- Insulin Syringe (U-100) for administration
Step 1: Clean and Prep
Pop the plastic caps off both the peptide vial and the bacteriostatic water vial. Thoroughly wipe the rubber stoppers of both vials with an alcohol prep pad. Let them air dry for 10 seconds.
Step 2: Equalize Pressure
Before drawing water, pull air into your mixing syringe equal to the amount of water you plan to use (e.g., if using 2mL of water, pull 2mL of air). Inject this air into the bacteriostatic water vial. This creates positive pressure, making it easier to draw the liquid out.
Step 3: Draw the Water
Flip the bacteriostatic water vial upside down and draw the desired amount of water (e.g., 1mL, 2mL, or 3mL) into the syringe.
Step 4: Inject Slowly
Insert the needle into the center of the peptide vial. Aim the needle tip toward the glass wall of the vial, not directly at the powder. Slowly inject the water so it trickles down the glass.
Important: Peptides are fragile chains of amino acids. Shooting water directly onto the powder can damage the bonds.
Step 5: Dissolve Gently
Once the water is added, remove the syringe. Do not shake the vial. Instead, gently swirl the vial in a circular motion until all the powder is dissolved and the solution is clear.
Understanding the Math (The "Peptide Math")
If you want to double-check the calculator, here is the formula used for reconstitution:
Know your concentration: If you have a 5mg vial and you add 2mL of water, the concentration is 2.5mg per mL.
Convert to micrograms: Since 1mg = 1000mcg, your solution is 2500mcg per mL.
Calculate the units: On a standard U-100 insulin syringe, 1mL = 100 units.
Therefore: 100 units = 2500mcg. 1 unit = 25mcg.
If your research subject requires a 250mcg dose, you would draw 10 units on the syringe.
Frequently Asked Questions (FAQ)
How to calculate reconstitution of peptide?
To calculate peptide reconstitution, divide the total peptide amount (in mg) by the volume of bacteriostatic water you add (in mL) to get your concentration. For example, 5mg of peptide in 2mL of water = 2.5mg/mL (or 2500mcg/mL). Then divide your desired dose by the concentration to find your injection volume. Use the calculator above to do this math instantly.
How much water to mix with peptides?
The amount of bacteriostatic water depends on your desired concentration and dose convenience. Common amounts are 1mL, 2mL, or 3mL per vial. Less water = higher concentration (smaller injection volume). More water = lower concentration (easier to measure small doses). Most people use 1-2mL for a 5mg vial or 2-3mL for a 10mg vial.
How much bacteriostatic water to mix with 10mg of BPC-157?
For a 10mg BPC-157 vial, adding 2mL of bacteriostatic water gives you a concentration of 5mg/mL (5000mcg/mL). At a common dose of 250mcg, you would draw 5 units on a U-100 insulin syringe. Adding 1mL would give 10mg/mL (2.5 units per 250mcg dose), while 3mL gives ~3.33mg/mL (7.5 units per dose).
How do I reconstitute peptides?
Clean both vial stoppers with alcohol. Draw bacteriostatic water into a syringe, then slowly inject it into the peptide vial—aim the needle at the glass wall, letting the water trickle down gently. Never spray directly onto the powder. Once added, gently swirl (don't shake) until the powder fully dissolves. Store refrigerated at 36-46°F.
What is the difference between Bacteriostatic Water and Sterile Water?
Bacteriostatic water contains 0.9% benzyl alcohol, which acts as a preservative to prevent bacterial growth. This allows the vial to be used multiple times over several weeks. Sterile water does not contain alcohol and should generally be used only for single-use vials, as bacteria can grow once the seal is broken.
Does BPC-157 need to be refrigerated?
Yes. Once reconstituted with bacteriostatic water, almost all peptides (including BPC-157, CJC-1295, and GLP-1 agonists) must be stored in the refrigerator (36°F - 46°F) to maintain stability. They typically remain stable for 4–6 weeks depending on the specific compound.
Why is my peptide solution cloudy?
Most peptides should result in a clear solution. If the solution remains cloudy or has floating particles after swirling, let it sit for a few minutes. If it does not dissolve, the peptide may be degraded, or the purity may be low.
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Medical Disclaimer: This calculator is for educational and research purposes only. It is not intended to provide medical advice, diagnosis, or treatment. Hunter Williams and Fully Optimized Health are not medical providers. The use of peptides and research chemicals carries risks. Always consult with a licensed healthcare professional before handling or administering any compound. The user assumes full responsibility for the accuracy of calculations and the use of any materials.